WebA protocol for the isolation and cultivation of brown bear (Ursus arctos) adipocytes Cytotechnology. 2016 Oct;68(5):2177-91. doi: 10.1007/s10616-015-9937-y. Epub 2016 Feb 8. Authors ... Differentiation conditions were optimized with respect to fetal bovine serum content and time spent in differentiation medium. Cultures were characterized ... WebNov 18, 2013 · The aim of this study was to provide an optimized protocol for isolating total RNA from adipose tissue. This was achieved by combining the advantages of the two routinely used methods, TRI Reagent® and miRNeasy. ... Isolation of total RNA and detection procedures for miRNA present in bovine-cultured adipocytes and adipose tissues. …
Brown Adipose Tissue Heterogeneity, Energy Metabolism, and Beyond
WebHere, we report an optimized PDGF-AB/AZA treatment protocol that was used to convert primary human adipocytes, a tissue source that is easily accessible and requires minimal manipulation, from adult donors aged 27 to 66 years into iMS cells with long-term repopulating capacity and multilineage differentiation potential. WebThe pre-adipocyte 3T3-L1 cell culture line, derived from mouse embryos, is a widely used ... In this protocol, differentiation is achieved in vitro through the sequential application of adipogenic factors to 3TC-L1 cells over a period of several days in culture. Following ... must be optimized prior to running the stress test. Follow the ... sonew
Cell Press: STAR Protocols
WebAdipocytes are “smart” cells able within the tissue to communicate with surrounding cells, but also with various organs, particularly via leptin acting on the central nervous system. Brown adipose tissue (BAT) and white adipose tissue (WAT) are known to be distinct tissues, whereas the heterogeneity of WAT depots is well est- lished. WebMar 19, 2024 · We present a detailed protocol for gene editing in adipocytes using the … WebNov 1, 2013 · While ChIP protocols have been optimized for use with specific cell types and tissues including adipose tissue-derived cells, a working ChIP protocol addressing the challenges imposed by fresh whole human adipose tissue has not been described. ... (16, 20) or adipocyte-like cell lines . Yet, a working ChIP protocol for whole adipose tissue (AT ... soneva owner