WebThis study investigated the effect of type 1 gonadotropin releasing hormone receptor (GnRH-R) localization within lipid rafts on the properties of plasma membrane (PM) … WebJan 16, 2014 · Fluorescent molecular probes, which make these lipid nanostructures visible through optical techniques, are one of the tools currently used to study lipid rafts. The most common are lipophilic fluorescent probes that partition specifically into liquid … On the binary SM/DOPC axis of the phase diagram, coexistence is observed from … Fig. 2 shows how the exchange is affected by the concentration of mβCD and … However, when fluorescent microscopy has been used to examine the distribution of … As indicated, fluorescent lipid probes were added only to the lipid mixture forming … On the other hand, only chain-labeled lipid analogs are the probes of choice in case … We review structure and dynamic measurements of biomembranes by … Laurdan is a fluorescent membrane marker used to investigate membrane fluidity … Using fluorescence lifetime microscopy we study the structure of lipid domains in … This lipid exhibits a shift in its fluorescence emission maximum from green … Another, related possibility is that lipid-lipid interactions are strong enough at …
Lighting up the changes of plasma membranes during apoptosis …
WebThe fluorescence spectrum of the probe laurdan, which incorporates into the lipid phase in the membrane, is correlated to its physical state. Two-photon infrared excitation techniques have been successfully applied to detect laurdan emission. WebSimilarly, proteins that are associated with lipid rafts may require lipid environments that are enriched with cholesterol and glycosphingolipids. Sometimes, ... The fluorescent probe laurdan provides a simple solution for monitoring changes in the phase properties of the nanodisc lipid bilayer. dichotomous relationship definition
Sphingomyelin distribution in lipid rafts of artificial monolayer ...
WebPralle, A., Keller, P., Florin, E. L., Simons, K., and Horber, J. K. (2000) Sphingolipid-cholesterol rafts diffuse as small entities in the plasma membrane of mammalian cells. J. Cell Biol.148, 997–1008. Bergelson, L. D., Molotkovsky, J. G., and Manevich, Y M. (1985) Lipid-specific fluorescent-probes in studies of biological-membranes. Chem. Phys. WebApr 1, 2004 · Furthermore, a green fluorescent protein-D4 fusion protein (GFP-D4) was revealed to be useful for real-time monitoring of cholesterol in lipid rafts in the plasma membrane. In addition, the expression of GFP-D4 in the cytoplasm might allow the investigations of intracellular trafficking of lipid rafts. WebDec 22, 2024 · Abstract Lipid droplets (LDs) have been known as a non-negligible cellular organelle for lipid storage and metabolism. Fluorescent probes for imaging LDs would … dichotomous research