Cisplatin p53
WebMay 7, 2024 · Consistently, genetic depletion or pharmacological inhibition of USP10 dramatically reduces the growth of lung cancer xenografts lacking wild-type p53 and sensitizes them to cisplatin.... WebThe inhibition of p53 by pifithrin-α attenuated the cisplatin-induced kidney injury and up-regulated miR-142-5p expression. We also identified the Sirtuin7 (SIRT7) as a target of miR-142-5p.
Cisplatin p53
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WebOct 9, 2024 · However, the cisplatin resistance of cells associated with p53 and RAS status was quite different because the inhibition of STAT3 without regard of RAS V12 reduced the IC50 of cells to cispaltin ... WebJul 31, 2014 · To confirm that p53 acted as a mediator of cisplatin-induced nephrotoxicity, we first assessed renal tubular damage by histology scores following 3 days of cisplatin administration in p53-null mice (p53 −/−). C57BL/6 mice, the most commonly used strain for p53 knockout, are relatively resistant to cisplatin-induced nephrotoxicity compared ...
Webp53 mediates cisplatin-induced apoptosis in renal proximal tubular cells, and p53 can activate caspase-3 . Related to this, Li et al. described that HRPTEp cells treated with DDP for 48 h showed that DDP led to significantly upregulated miR-449a. In the same way, the overexpression of miR-449a led to an increased apoptotic rate of HRPTEpCs ... WebMay 16, 2007 · Cisplatin induces p53 mitochondrial translocation: Effect of pifithrin-α. HCT116 wt cells (pre-treated or not with 10 μM pifithrin-α for 16 h) were treated with 50 μM cisplatin for 30 min or maintained untreated. Then, mitochondrial and cytosolic ( A) or total cell extracts ( B) were obtained.
WebWhen p53 was inhibited by 20 μM pifithrin-α (PFT-α) (Sigma-Aldrich) in cisplatin-treated cells and the expression levels of glycolysis-related genes were determined by qRT-PCR ( Fig 5 B), it... WebMay 26, 1998 · Cisplatin treatment of teratocarcinoma cells with a wild-type p53 gene resulted in accumulation of the p53 protein through posttranscriptional mechanisms; induction of p53-target genes was also observed. Drug treatment resulted in rapid apoptosis in p53-wild-type cells but not in p53 −/− teratocarcinoma cells.
WebDec 1, 2004 · Tubular damage by cisplatin leads to acute renal failure, which limits its use in cancer therapy. In tubular cells, a primary target for cisplatin is presumably the genomic DNA. However, the pathwa... Role …
WebOct 28, 2024 · The mutational status of p53 seems to be related to the preferential response of tumor cells to Wee1 inhibitors 21 – 23. Studies have shown that AZD1775 radiosensitized p53-defective human cells derived from lung, breast, and prostate cancers by abrogating the radiation-induced G2 block, while this was not observed in p53 wild-type cells 24 ... graphing factorsWebFeb 24, 2003 · Because p53 is induced by cisplatin in HCT116 cells (Fig. 1), it could have accounted for this apoptosis response. Transfection with PMS2 did not increase the apoptosis response to cisplatin. In p73-transfected cells, cisplatin did not stimulate apoptosis caused by the overexpression of p73. This is consistent with the inability of … chirping summer insectWebJul 1, 2024 · 1. Introduction. Cisplatin is widely and commonly used as a chemotherapeutic agent for the treatment of solid tumors, but the frequent occurrence of renal injury is the major limitation of cisplatin-based chemotherapy [1, 2].Renal proximal tubular damage due to activation of cell death and inflammatory pathways pathophysiologically characterizes … chirping the cameramanWebJan 30, 2024 · (D) Mechanism that CKI sensitizes p53-mutant CRC cells to cisplatin. The R273H/P309S mutation of p53 rendered CRC cells insensitive to Cis and 5FU treatments. The in vitro anti-CRC profiles of CKI + Cis combination were distinct from that of CKI+5FU. CKI decreased drug sensitivity to 5FU in both p53 wild and R273H/P309S mutation CRC … chirping text messagingWebJul 19, 2024 · Background Non-small cell lung cancer (NSCLC) is the leading cause of cancer deaths primarily due to chemoresistance. Somatic mutation of TP53 (36%) and epidermal growth factor receptor (EGFR; > 30%) are major contributors to cisplatin (CDDP) resistance. Substantial evidence suggests the elevated levels of reactive oxygen species … chirping sugar gliderWebJan 1, 2024 · The p53 and p21 proteins, which are senescence modulators, were upregulated at 48 h after cisplatin treatment ( Fig. 1 E and F). The mRNA levels of senescence-associated secretory phenotype (SASP) factors including interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α were increased in NRK cells at 48 h after … chirping tales mukteshwarWebCisplatin inhibited glycolysis via p53 activation. (A) Western blot analysis indicated increased levels of p53 and phospho-p53 after 6 and 24 h of cisplatin treatment. The … graphing feasible region calculator