WebThermo Scientific Eco52I (EagI) restriction enzyme recognizes C^GGCCG sites and cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme ... WebAn E. coli strain that carries the ApeKI gene from Aeropyrum pernix K1 (ATCC 700893). This product is related to the following categories: Restriction Endonucleases: A, Time-Saver Qualified Restriction Enzymes Products. This product can be used in the following applications: Fast Cloning: Accelerate your cloning workflows with reagents from NEB,

DNA methylation regulates p27kip1 expression in rodent

WebNew England Biolabs avai enzyme pcr product neb buffer h 2 o Avai Enzyme Pcr Product Neb Buffer H 2 O, supplied by New England Biolabs, used in various techniques. Bioz … WebEnzyme gene sequenced Enz Comment: AvaI seq 1 to 471 bp one strand. R gene expression for AvaI is GTG 3023 bp to TGA 3967 bp, however Met overlapping with M.AvaI at 2996 could be R.AvaI start. Alan Friedman collaborating on crystal structure. Molecular Weight: 35676 # sites on Adeno2: 40 Lambda: 8 homesecuur

BsoBI NEB

WebOne unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in 50 µl of reaction buffer. Reaction Conditions. 1X rCutSmart™ Buffer Incubate at 37°C . 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate WebAvaI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10137108. ... One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Reaction Conditions. 1X … WebThe restriction enzyme AvaI from the blue-green algaeAnabaena variabili s (3) is an isoschizomer ofBs oBI. Previ-ously, we had preliminarily demo- n strated SDA at 37°C … hip hop pase